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pb25 44 01 (PCR Biosystems Ltd)

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PCR Biosystems Ltd pb25 44 01
Pb25 44 01, supplied by PCR Biosystems Ltd, used in various techniques. Bioz Stars score: 95/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pb25+44+01/qPCRBIO+Probe+1-Step+Go+Lo-ROX/pmc12966372-321-19-20
Average 95 stars, based on 57 article reviews

pb25 44 01 - by Bioz Stars, 2026-09

95/100 stars

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Article Title: miR-424(322) ~ 503 impairs colon cancer progression driven by PTEN deficiency
Article Snippet: .. Quantitative real-time PCR was performed with 50 ng of total RNA using the one-step protocol qPCRBIO Probe 1-step Go (PB25.44-01, PCR Biosystems) according to manufacturer’s protocol. .. Primers used for gene expression assays were commercially obtained from Applied Biosystems and are listed in Supplementary Materials (Table ).

Article Title: miR-424(322) ~ 503 impairs colon cancer progression driven by PTEN deficiency.
Article Snippet: .. Quantitative real-time PCR was performed with 50ng of total RNA using the one-step protocol qPCRBIO Probe 1-step Go (PB25.44-01, PCR Biosystems) according to manufacturer’s protocol. .. Primers used for gene expression assays were commercially obtained from Applied Biosystems and are listed in Supplementary Materials (Table S4).

Article Title: Endometrial cancer progression driven by PTEN-deficiency requires miR-424(322) ∼ 503
Article Snippet: Total RNA from organoid cultures was extracted using SurePrep TrueTotal RNA Purification Kit (BP2800-50, Fisher BioReagents) according to manufacturer’s protocol. .. Quantitative real-time PCR was performed with 50ηg of total RNA using the one-step protocol qPCRBIO Probe 1-Step Go (PB25.44-01, PCR Biosystems). .. Primers used for gene expression analysis were commercially obtained from Applied Biosystems: Ccnd1 (Mm00432359_m1) and Gapdh (Mm99999915_g1).

Article Title: Endometrial cancer progression driven by PTEN-deficiency requires miR-424(322) ~ 503
Article Snippet: Total RNA from organoid cultures was extracted using SurePrep TrueTotal RNA Purification Kit (BP2800-50, Fisher BioReagents) according to manufacturer’s protocol. .. Quantitative real-time PCR was performed with 50ηg of total RNA using the one-step protocol qPCRBIO Probe 1-Step Go (PB25.44-01, PCR Biosystems). .. Primers used for gene expression analysis were commercially obtained from Applied Biosystems: Ccnd1 (Mm00432359_m1) and Gapdh (Mm99999915_g1).

Article Title: Endometrial cancer progression driven by PTEN-deficiency requires miR-424(322) ~ 503.
Article Snippet: Total RNA extraction, real-time qPCR Total RNA from organoid cultures was extracted using SurePrep TrueTotal RNA Purification Kit (BP2800-50, Fisher BioReagents) according to manufacturer’s protocol. .. Quantitative real-time PCR was performed with 50ηg of total RNA using the one-step protocol qPCRBIO Probe 1-Step Go (PB25.44-01, PCR Biosystems). .. Primers used for gene expression analysis were commercially obtained from Applied Biosystems: Ccnd1 (Mm00432359_m1) and Gapdh (Mm99999915_g1).

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$Establishment of ViREn. ( A ) Schematics of the ViREn workflow. DENV RNA from either infected cells or virions was separated by sucrose gradient ultracentrifugation. DENV gRNA and sfRNA were further enriched using antisense affinity capture. The purified DENV gRNA was subjected to RNA sequencing-based approaches for the identification and mapping of m 5 C modifications. The distribution of RNAs extracted from infected cells ( B ) and virions ( C ) across sucrose density gradient fractions was analyzed by denaturing agarose gel and corresponding northern blot using a hybridization probe targeting DENV 3′UTR. The percentage of DENV gRNA in each fraction is shown at the bottom (mean ± SD, n = 3). ( D ) DENV gRNA collected in fraction 11 was further purified by affinity capture. Its integrity was visualized by northern blotting. ( E ) The level of DENV gRNA and 28S rRNA contaminant, before and after affinity capture, was measured by probe-based RT-qPCR (mean ± SD, n = 3). ( F ) The total RNA composition of fraction 11 before and after affinity capture was analyzed by Illumina-based RNA-seq ( n = 3). Shown is the proportion of reads in each sample corresponding to DENV gRNA, human genome, rRNAs, and unmapped reads (mean ± SD, n = 3).$

Journal: Nucleic Acids Research

Article Title: The two-step purification method ViREn identifies a single NSUN6-mediated 5-methylcytosine modification promoting dengue virus RNA genome turnover

doi: 10.1093/nar/gkag003

Figure Lengend Snippet: Establishment of ViREn. ( A ) Schematics of the ViREn workflow. DENV RNA from either infected cells or virions was separated by sucrose gradient ultracentrifugation. DENV gRNA and sfRNA were further enriched using antisense affinity capture. The purified DENV gRNA was subjected to RNA sequencing-based approaches for the identification and mapping of m 5 C modifications. The distribution of RNAs extracted from infected cells ( B ) and virions ( C ) across sucrose density gradient fractions was analyzed by denaturing agarose gel and corresponding northern blot using a hybridization probe targeting DENV 3′UTR. The percentage of DENV gRNA in each fraction is shown at the bottom (mean ± SD, n = 3). ( D ) DENV gRNA collected in fraction 11 was further purified by affinity capture. Its integrity was visualized by northern blotting. ( E ) The level of DENV gRNA and 28S rRNA contaminant, before and after affinity capture, was measured by probe-based RT-qPCR (mean ± SD, n = 3). ( F ) The total RNA composition of fraction 11 before and after affinity capture was analyzed by Illumina-based RNA-seq ( n = 3). Shown is the proportion of reads in each sample corresponding to DENV gRNA, human genome, rRNAs, and unmapped reads (mean ± SD, n = 3).

Article Snippet: Briefly, 3 μl of extracted RNA were mixed with 12 μl qPCR mix (qPCRBIO Probe 1-Step Go Lo-ROX, PCR Biosystems) according to the manufacturer instructions and using the following cycling protocol: 50°C 10 min, 95°C 1 min, 95°C 10 s, 60°C 1 min; steps 3–4 were repeated 39 times.

Techniques: Infection, Purification, RNA Sequencing, Agarose Gel Electrophoresis, Northern Blot, Hybridization, Quantitative RT-PCR